The Lineweaver-Burk plot is a graphical illustration of the Michaelis-Menten equation, which describes the connection between the response velocity and the substrate focus in an enzyme-catalyzed response. The preliminary velocity is the rate of the response at first, when the substrate focus is zero. To search out the preliminary velocity on a Lineweaver-Burk plot, you’ll be able to extrapolate the linear portion of the curve to the y-axis. The y-intercept of this line provides you with the preliminary velocity.
The Lineweaver-Burk plot is a great tool for figuring out the kinetic parameters of an enzyme-catalyzed response. The Michaelis fixed (Km) is the substrate focus at which the response velocity is half of the utmost velocity. The Vmax is the utmost velocity of the response. The Lineweaver-Burk plot can be used to find out the kind of inhibition that’s occurring in an enzyme-catalyzed response.
